Jessica Prenni, Ph.D.

jessica prenniDirector, Proteomics and Metabolomics Facility

Associate Professor, Department of Biochemistry and Molecular Biology

Office: Microbiology C123

Phone: 970-491-0961


Education and Training

B.S. Chemistry, 1998, Southern Oregon University, Ashland OR

Ph.D. Analytical Chemistry, 2002, University of Colorado, Boulder CO

Post-doctoral Scientist, 2002-2003, Scripps Research Institute, La Jolla, CA

Post-doctoral Scientist, 2004-2006, Colorado State University, Fort Collins, CO


Dr. Prenni has extensive experience (11 years) in the identification and characterization of biological molecules by mass spectrometry.  During her post-doctoral appointment in the Mass Spectrometry Facility at the Scripps Research Institute in La Jolla, CA she focused on the evaluation and optimization of a new ionization technique involving matrix-free desorption on silicon. During her time at CSU she has been responsible for the establishment of the Proteomics and Metabolomics Facility and has worked to promote the use of shared resources and expertise for the CSU research community.

In addition to her role at CSU, she participates as an instructor in a very successful hands-on Proteomics and Metabolomics training program through National Jewish Health in Denver, CO ( She is also co-founder and president elect of the Colorado Biological Mass Spectrometry Society (


Google Scholar

Brandt LE, Ehrhart EJ, Scherman H, Olver CS, Bohn AA, Prenni JE 2014 “Characterization of the canine urinary proteome”  Veterinary Clinical Pathology 1-14.

Henley, T., Reddivari, L., Broeckling, C.D.l, Bunnng, M., Miller, J., Avens, J.S., Stone, M., Prenni, J.E. & Vanamala, J. 2014 “American India Pale Ale matrix rich in xanthohumol is potent in suppressing proliferation and elevating apoptosis of human colon cancer cells” International Journal of Food Science and Technology May10, DOI: 10.1111/ijfs.12570.

Kumar J, Karlsson R, Broeckling CD, Prince JA, Hong MG, Prenni JE, Ingelsson E, Wiklund F 2014 “Associations of Body Mass Index and Obesity-Related Genetic Variants with Serum Metabolites” Current Metabolomics 2(1), 27-36. DOI: 10.2174/2213235X02666140214200752.

Barnes B, Wilson M, Carr P, Vitha M, Broeckling C, Heuberger A, Prenni J, Gregory J, Corcoran H, Snow N, Chopra S, Dhandapani R, Tawfall A, Sumner L, Boswell P, 2013 “ “Retention Projection” Enables Reliable Use of Shared Gas Chromatographic Retention Data Across Labs, Instruments, and Methods” Analytical Chemistry, 85(23): 11650-11657.

Reisdorph N, Stearman R, Kechris K, Phang TL, Reisdorph R, Prenni J, Erle DJ, Coldren C, Schey K, Nesvizhskii A, Geraci M, 2014 “Hands on Workshops as an Effective Means of Learning Advanced Technologies Including Genomics, Proteomics, and Bioinformatics”  Genomics, Proteomics, and Bioinformatics,  Dec;11(6):368-77.

Webb K, Broccardo C, Prenni JE, Wintermantel W. 2014 “Proteomics Profiling of Sugar Beet (Beta vulgaris) Leaves during Rhizomania Compatible Interactions” Proteomes  2(2), 208-223.

Heuberger, A.L., Broeckling, C.D., Kirkpatrick K.R. and Prenni J.E. (2013) Application of nontargeted metabolite profiling to discover novel markers of quality traits in an advanced population of malting barley. Plant Biotechnol. J., doi: 10.1111/pbi.12122.

Schauer KL, Freund DM, Prenni JE, Curthoys NP, 2013 “Proteomics Profiling and Pathway Analysis of the Response of Rat Renal Proximal Convoluted Tubules to Metabolic Acidosis” AJP - Renal Physiology Sep 1;305(5):F628-40.

Broccardo, CJ, Hussey GS, Goehring L, Lunn P, Prenni JE 2013“Proteomic Characterization of Equine Cerbrospinal Fluid” Journal of Equine Veterinary Science, 34(3) 451-458.

Schauer, KL, Broccardo CJ, Webb KM, Covey PA, Prenni JE 2013 “Mass Spectrometry Contamination from Tinuvin 770, a Common Additive in Laboratory Plastics” Journal of Biomolecular Techniques, July 2402-004.

Freund DM, Prenni JE, Curthoys NP 2013 “Proteomics Profiling of the Mitochondrial Inner Membrane of Rat Renal Proximal Convoluted Tubules” Proteomics  Aug;13(16):2495-9.

Broccardo CJ, Schauer KL, Kohrt WM, Schwartz RS, Murphy JP, Prenni JE 2013 “Multiplexed Analysis of Steroid Hormones in Human Serum Using Novel Microflow Tile Technology and LC-MS/MS” Journal of Chromatography B Sep 1;934:16-21.

Kumar J, Broeckling CD, Wiklund F, Ingelsson E, Prenni JE, 2013 “Influence of Biological and Technical Covariates on Non-targeted Metabolite Profiling in a Large-scale Epidemiological Study” Current Metabolomics, 1(3):220-226.

Ryan EP, Heuberger AL, Broeckling CD, Borresen E, Oillia C, Prenni JE 2013 “Advances in Nutritional Metabolomics” Current Metabolomics, 1(2):109-120.

Shen, M, Broeckling CD, Chu EY, Ziegler G, Baxter IR, Prenni JE, Hoekenga OA, 2013 “Leveraging non-targeted metabolite profiling via statistical genomics” PLoS ONE 8(2): e57667. doi:10.1371/journal.pone.0057667.

Fruend DM, Prenni JE 2013 "Improved Detection of Quantitative Differences Using a Combination of Spectral Counting and MS/MS Total Ion Current” Journal of Proteome Research, 12 (4), pp 1996–2004.

Kalashnikova AA, Duane WD, McBryant SJ, Herman JA, Deluca J, Luger K, Prenni JE, Hansen JC 2013 "Linker Histone H1.0 Interacts with an Extensive Network of Proteins Involved in mRNA Metabolism" Nucleic Acids Research, April 1;41(7):4026-35.

Broeckling CD, Heuberger AL, Prenni JE 2013 “Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS)” J Vis Exp. Mar 14;(73):e50242.

Hong, MG, Karlsson R, Magnusson PKE, Lewis MR, Isaacs W, Zheng LS, Xu J, Gronberg H, Ingelsson E, Pawitan Y, Broeckling CD, Prenni JE, Wiklund F, Prince JA 2012 “A Genome-Wide Assesment of Variability in Human Serum Metabolism” Human Mutation, Mar;34(3)”515-24.

Prenni AJ, Tobo1 Y, Garcia E, DeMott PJ, McCluskey CS, Kreidenweis SM, Prenni JE, Huffman JA, Pöhlker C, Pöschl U 2012 “The impact of rain on ice nuclei populations at a forested site in Colorado” Geophysical Research Letters doi:10.1029/2012GL053953.

Freund DM, Prenni JE, Curthoys NP 2012 “Response of the Mitochondrial Proteome of Rat Renal Proximal Convoluted Tubules to Chronic Metabolic Acidosis” AJP - Renal Physiology  Jan;304(2);F145-55.

Bisson GP, Mehaffy C, Broeckling C, Prenni J, Rifat D, Lun DS, Burgos M, Weissman D, Karakousis PC, Dobos K., 2012 “Upregulation of the phthiocerol dimycocerosate biosynthetic pathway by rifampicin-resistant, rpoB-mutant Mycobacterium tuberculosis.” J Bacteriol. 2012 Sep 21

Broeckling, C.D. Heuberger, A.L., Prince, J.A., Ingelsson, E., Prenni, J.E., 2012, “Assigning precursor-product ion relationships in indiscriminant MS/MS data from non-targeted metabolite profiling studies.” Metabolomics, 9:33-43.

Heuberger AL, Broeckling C, Lewis M, Salazar L, Bouckaert P, Prenni, JE., 2012,Metabolomic profiling of beer reveals effect of temperature on non-volatile small molecules during short-term storage.” Food Chemistry, Dec 1;135(3):1284-9.

Prenni JE, Vidal, M., Olver C.S. 2012, “Preliminary Characterization of the murine memrane reticulocyte proteome”. Blood Cells, Molecules, and Diseases, Aug 15;49(2):74-82.

Cho, YS, Dobos KM, Prenni JE, Yang H, Hess A, Rosenkrands I, Andersen P, Ryoo SW, Bai GH, Brennan MJ, Izzo A, Bielefeldt-Ohmann H, Belisle JT*, 2012, “Deciphering the proteome of the in vivo diagnostic reagent “purified protein derivative” from Mycobacterium tuberculosisProteomics 2012, 12, 979–991.

Fujimoto S; Seebart C;  Guastafierro T; Prenni J;  Caiafa P; Zlatanova J “Proteome analysis of protein partners to nucleosomes containing canonical H2A or the variant histones H2A.Z or H2A.X" 2012 Biological Chemistry, Jan 1;393(1-2):47-61.

Machain-Williams C., Mammen M.P., Zeidner N.S, Beaty B.J., Prenni J.E., Nisalak A., Blair C.D. 2011, “Association of Human Immune Response to Aedes aegypti Salivary Proteins with Dengue Disease Severity”, Parasite Immunology, 34(1): 15-22.

Ryan E.P.; Heuberger A.L.; Weir T.L.; Barnett B.; Broeckling C.D.; Prenni J.E. 2011 “Rice Bran Fermented with Saccharomyces boulardii Generates Novel Metabolite Profiles with Bioactivity.” J Agric Food Chem. 59(5): 1862–1870.

Goldstrohm  D.A.; Broeckling C.D.; Prenni J.E.; Curthoys N.P 2010 “Importance of Manual Validation for the Identification of Phosphopeptides using a Linear Ion Trap Mass Spectrometer” J  Biomol Tech., 22(1): 10–20.

Seebart, C.; Prenni, JE.; Tomschik, M.; Zlatanova, J. 2010 “New Nuclear Partners for Nucleosome Assembly Protein 1:  Unexpected Associations” Biochem. Cell Biol. 88(6): 927-936.

Kruh, N.; Troudt, J.; Izzo, A.; Prenni, JE; Dobos, KM. 2010 "Portrait of a Pathogen: The Mycobacterium tuberculosis Proteome In VivoPLoS One. Nov 11;5(11): e13938.

Szerlong HJ; Prenni JE; Nyborg JK; Hansen JC. 2010 “Activator-dependent p300 acetylation of chromatin in vitro: enhancement of transcription by disruption of repressive nucleosome-nucleosome interations.” J Biol Chem. Aug 18.

Mehaffy C; Hess A; Prenni, JE; Mathema B; Kreiswirth B; Dobos KM 2010 "Descriptive proteomic analysis shows protein variability between closely related clinical isolates of Mycobacterium tuberculosis" Proteomics, Mar 9;10(10):1966-1984.

Wamsley S; Broeckling C; Hess A; Prenni JE; Curthoys N 2010 "Proteomic Analysis of Brush Border Membrane Vesicles Isolated from Purified Proximal Convoluted Tubules" Am J Physiol Renal Physiol, 298(6):F1323-31.

Mueller NH; Walters MS; Marcus R; Graf LL; Prenni JE; Gilden D; Silverstein, S.J.; Cohrs, R.J. 2010 “Identification of Phosphorylated Residues on Varicella-Zoster Virus Immediate Early Protein ORF63” J Gen Virol, May;91(Pt 5):1133-7.

Olver CS; Webb TL; Long LJ; Scherman H; Prenni JE 2010 “Comparison of methods for albumin and immunoglobulin depletion from equine serum.” Veterinary Clinical Pathology, Sep;39(3):337-45.

Okaysu, E; Freund DM; Prenni JE; Curthoys NP (2010) “Elucidation of Phosphoproteins Involved in the Renal Cellular Response to Acute Metabolic Acidosis” Journal of Undergraduate Research and Scholarly Excellence, 14-19.

Bhamidi S; Scherman MS; Rithner CD; Prenni JE; Chatterjee D; Khoo K; and McNeil MR 2008 “The Identification and Location of Succinyl Residues and the Characterization of the Interior Arabinan Region Allows for a Model of the Complete Primary Structure of Mycobacterium Tuberculosis Mycolyl Arabinogalactan” Journal of Biological Chemistry, 283(19), 12992-13000.

Dooley GP; Reardon KF; Prenni JE; Legare ME; Foradori CD; Tessari JE; and Hanneman WH 2009 “Proteomic Analysis of Diaminochlorotriazine (DACT) Adducts in Wister Rat Pituitary Glands and LbetaT2 Rat Pituitary Cells”  Chemical Research in Toxicology, 21(4), 844-851.

Prenni JE; Swardson-Olver CJ 2007 “Proteomics: A Review and an Illustration” Veterinary Clinical Pathology, 36(1) 13-24.

Bruemmer J; Lewis M; Prenni JE; Squires E; Hansen T (2007) “Gene Expression in the Equine Endometrium during Maternal Recognition of Pregnancy” Biology of Reproduction, 77, 98-b-98.

Dooley GP; Prenni JE; Cranmer BK; Prentiss PL; Andersen ME; Tessari JD  2006 “Identification of a Novel Hemoglobin Adduct in Sprague Dawley Rats Exposed to Atrazine” Chemical Research In Toxicology, 19(5), 692-700.

Go EP; Prenni JE; Wei J; Jones A; Hall SC; Witkowska HE; Shen Z; Siuzdak G 2003 “Desporption/Ionization on Silicon Time-of-Flight/Time-of-Flight Mass Spectrometry” Analytical Chemistry, 75, 2504-2506.

Prenni JE; Shen Z; Trauger S; Chen W; Siuzdak G 2003 “Protein Characterization using Liquid Chromatography Desorption/Ionization on Silicon Mass Spectrometry (LC-DIOS-MS)” Spectroscopy, 17, 693-698.

*Ekhoff J.A.; Farrow M.J.; Walba D.M.; Rowlen K.L. 2003 “Molecular Orientation of a Model Liquid Crystal Alignment Layer” Talanta, 60, 801-808.

*Ekhoff J.A.; Rowlen K.L. 2002 “Effect of Interfacial Refractive Index on Optical Molecular Orientation Measurements” Analytical Chemistry, 74, 5954-5959.

*Ekhoff J.A.; Westerbuhr S.G.; Rowlen K.L. 2001 “Evidence of Spontaneous Multilayer Formation for Disperse Red-1 at a Fused-Silica/2-Propanol Interface”, Langmuir, 17, 7079-7084.

Espinoza EO; Cech-Lindly N; Gordon KM; *Ekhoff JA; Kirms MA 1999 “Electrospray Ionization Mass Spectrometric Analysis of Blood Differentiation of Species”, Analytical Biochemistry, 268, 252-261.

Vance F.W.; Lemon B.I.; *Ekhoff J.A.; Hupp J.T.; 1998 “Interrogation of Nanoscale Silicon Dioxide/Water Interfaces via Hyper-Rayleigh Scattering”, Journal of Physical Chemistry B, 102, 1846-1848.


*maiden name J.A. Ekhoff



© Copyright 2007, Colorado State University
Fort Collins, Colorado 80523
Maintained by Jessica Prenni