Jessica Prenni, Ph.D.
Director, Proteomics and Metabolomics Facility
Assistant Professor, Department of Biochemistry and Molecular Biology
Office: Microbiology C123
Phone: 970-491-0961
e-mail: jprenni@colostate.edu
Education and Training
B.S. Chemistry, 1998, Southern Oregon University, Ashland OR
Ph.D. Analytical Chemistry, 2002, University of Colorado, Boulder CO
Post-doctoral Scientist, 2002-2003, Scripps Research Institute, La Jolla, CA
Post-doctoral Scientist, 2004-2006, Colorado State University, Fort Collins, CO
Background
Dr. Prenni has extensive experience (9 years) in the identification and characterization of protein and peptides by mass spectrometry. During her post-doctoral appointment in the Mass Spectrometry Facility at the Scripps Research Institute in La Jolla, CA she focused on the evaluation and optimization of a new ionization technique involving matrix-free desorption on silicon. During her time at CSU she has been responsible for the establishment of the Proteomics and Metabolomics Facility and has worked to promote the use of shared resources and expertise for the CSU research community.
In addition to her role at CSU, she participates as an instructor in a very successful hands-on Proteomics and Metabolomics training program through National Jewish Health in Denver, CO (http://www.metabolomicstraining.org/). She is also co-founder and president of the Colorado Biological Mass Spectrometry Society (www.cbmss.org).
Select Publications
Espinoza EO; Cech-Lindly N; Gordon KM; Ekhoff* JA; Kirms MA “Electrospray Ionization Mass Spectrometric Analysis of Blood Differentiation of Species”, Analytical Biochemistry, 1999, 268, 252-261.
Go EP; Prenni JE; Wei J; Jones A; Hall SC; Witkowska HE; Shen Z; Siuzdak G “Desporption/Ionization on Silicon Time-of-Flight/Time-of-Flight Mass Spectrometry” Analytical Chemistry, 2003, 75, 2504-2506.
Prenni JE; Shen Z; Trauger S; Chen W; Siuzdak G “Protein Characterization using Liquid Chromatography Desorption/Ionization on Silicon Mass Spectrometry (LC-DIOS-MS)” Spectroscopy, 2003, 17, 693-698.
Dooley GP; Prenni JE; Cranmer BK; Prentiss PL; Andersen ME; Tessari JD “Identification of a Novel Hemoglobin Adduct in Sprague Dawley Rats Exposed to Atrazine” Chemical Research In Toxicology, 2006, 19(5), 692-700.
Prenni JE; Swardson-Olver CJ “Proteomics: A Review and an Illustration” Veterinary Clinical Pathology, 2007, 36(1) 13-24.
Dooley GP; Reardon KF; Prenni JE; Legare ME; Foradori CD; Tessari JE; and Hanneman WH “Proteomic Analysis of Diaminochlorotriazine (DACT) Adducts in Wister Rat Pituitary Glands and LbetaT2 Rat Pituitary Cells” Chemical Research in Toxicology, 2008, 21(4), 844-851.
Bhamidi S; Scherman MS; Rithner CD; Prenni JE; Chatterjee D; Khoo K; and McNeil MR “The Identification and Location of Succinyl Residues and the Characterization of the Interior Arabinan Region Allows for a Model of the Complete Primary Structure of Mycobacterium Tuberculosis Mycolyl Arabinogalactan” Journal of Biological Chemistry, 2008, 283(19), 12992-13000.
Olver CS; Webb TL; Long LJ; Scherman H; Prenni JE “Comparison of methods for albumin and immunoglobulin depletion from equine serum.” Veterinary Clinical Pathology, 2010, In Press.
Mueller NH; Walters MS; Marcus R; Graf LL; Prenni JE; Gilden D; Silverstein, S.J.; Cohrs, R.J. “Identification of Phosphorylated Residues on Varicella-Zoster Virus Immediate Early Protein ORF63” 2010, J Gen Virol, May;91(Pt 5):1133-7.
Wamsley S; Broeckling C; Hess A; Prenni JE; Curthoys N "Proteomic Analysis of Brush Border Membrane Vesicles Isolated from Purified Proximal Convoluted Tubules" 2010 Am J Physiol Renal Physiol, 298(6):F1323-31.
Mehaffy C; Hess A; Prenni, JE; Mathema B; Kreiswirth B; Dobos KM "Descriptive proteomic analysis shows protein variability between closely related clinical isolates of Mycobacterium tuberculosis" 2010 Proteomics, Mar 9;10(10):1966-1984.
Szerlong HJ; Prenni JE; Nyborg JK; Hansen JC. “Activator-dependent p300 acetylation of chromatin in vitro: enhancement of transcription by disruption of repressive nucleosome-nucleosome interations. 2010 J Biol Chem. Aug 18.
Kruh, N.; Troudt, J.; Izzo, A.; Prenni, JE; Dobos, KM. "Portrait of a Pathogen: The Mycobacterium tuberculosis Proteome In Vivo" 2010, PLoS One. Nov 11;5(11): e13938.
Seebart, C.; Prenni, JE.; Tomschik, M.; Zlatanova, J. “New Nuclear Partners for Nucleosome Assembly Protein 1: Unexpected Associations” 2010, Biochem. Cell Biol. 88(6): 927-936.
Goldstrohm D.A.; Broeckling C.D.; Prenni J.E.; Curthoys N.P “Importance of Manual Validation for the Identification of Phosphopeptides using a Linear Ion Trap Mass Spectrometer” 2010, J Biomol Tech., in press.
Ryan E.P.; Heuberger A.L.; Weir T.L.; Barnett B.; Broeckling C.D.; Prenni J.E. “Rice Bran Fermented with Saccharomyces boulardii Generates Novel Metabolite Profiles with Bioactivity.” 2011. J Agric Food Chem. in press.
Machain-Williams C., Mammen MP, Zeidner NS, Beaty BJ, Prenni JE, Nisalak A, Blair CD “Association of Human Immune Response to Aedes aegypti Salivary Proteins with Dengue Disease Severity”,Parasite Immunol 2011 Oct 13
*maiden name J.A. Ekhoff